Can Freshwater Harlequin Shrimp (Sulawesi) and Least Killifish (Heterandria) Live Together?

Freshwater Harlequin Shrimp (Sulawesi) is a peaceful species (0/10), while Least Killifish (Heterandria) is peaceful (1/10). This modest difference means Least Killifish (Heterandria) may occasionally assert dominance over Freshwater Harlequin Shrimp (Sulawesi).

Freshwater Harlequin Shrimp (Sulawesi) and Least Killifish (Heterandria) both frequent the Bottom (Substrate) region of the setup. This overlap can increase competition for space, so providing ample room and cover is important to keep both species comfortable.

Worth noting: Freshwater Harlequin Shrimp (Sulawesi) is nocturnal and will be most active after lights-out, naturally reducing daytime encounters.

The safe temperature window for both species falls between 26°C and 27°C. Aim for the midpoint of this range (around 26.5°C) for optimal comfort.

Both fish can tolerate a pH between 7.5 and 8. Maintaining a stable value within this band is more important than hitting an exact number.

For general hardness, the shared comfort zone is 5–8 dGH. Regular testing will ensure conditions stay within this range.

To house Freshwater Harlequin Shrimp (Sulawesi) and Least Killifish (Heterandria) together, plan for an aquarium of at least 38 litres with a minimum length of 45 cm. This recommendation combines the stricter species minimums with a community-load allowance so the pairing has realistic long-term space and filtration headroom.

Because this pairing requires extra vigilance, include plenty of hiding spots — caves, driftwood, and dense plant clusters — so the less dominant fish can retreat when needed.

Specific environmental needs for this combination include: Established Algae (Otocinclus), Smooth Gravel (Sensitive Barbels), Plants - Densely covered, Plants - Floating. Meeting these requirements will help both species thrive.

Both species do well with low (still water) water movement, so a standard filtration setup rated for the tank volume should suffice.

This pairing usually fails when the tank is treated like a standard mixed community and the caution flags are ignored. Freshwater Harlequin Shrimp (Sulawesi) and Least Killifish (Heterandria) need extra room, more cover, and closer observation during the first few weeks so small aggression, feeding, or territory issues do not snowball into a long-term problem.